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KMID : 1134820000290040606
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Journal of the Korean Society of Food Science and Nutrition
2000 Volume.29 No. 4 p.606 ~ p.614
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Randomly Amplified Polymorphic DNA Analysis of Listeria Species Isolated from Foods in Korea
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Choi Young-Chun
Park Boo-Kil
Lee Tax-Soo
Oh Deog-Hwan
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Abstract
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This study was carried out for comparing Listeria strains developing genetic markers for Listeria strains using Listeria sp. genetic markers using Randomly Amplified Polymorphic DNA (RAPD) analysis method. Five of 10 RAPD primers (OPA-01, OP-26-01, OP-26-02, OPB-01, OP-26-10) showed the distinctive polymorphism among Listeria sp. isolated from domestic foods. RAPD-PCR with five arbitrary primers produced 76 DNA polymorphism. Among them, OPA-01 and OP-26-10 primers produced about 1.5 kb and 0.7 kb amplified DNA fragments for all the Listeria sp., but these amplified DNA fragment did not correspond to specific Listeria genus. The genetic relationships of Listeria sp. using NTSYS program were grouped into 7 clusters and showed 0.54 to 0.93 similarity among strains. Especially, No. 3 and No. 20 isolates showed genetically most similar relationship by 0.94, and No. 7 and No. 24, or No. 7 and No. 45 isolates showed the least similarity by 0.54. From these results, RAPD analysis method seemed to be successfully applied the classification and genetic analysis for Listeria sp. isolates.
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KEYWORD
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RAPD, Listeria sp., PCR
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